Utilization of reference genomes is possible because representatives of a species are genetically highly similar - for instance, any two human genome sequences are almost identical. The reconstruction of a genome can be facilitated if a reference genome is available to which the sequencing reads can be aligned. The continuous DNA sequence of a human genome can be computationally reconstructed by using overlaps between short sequencing reads. In comparison, most other personal genomics companies, including 23andMe and AncestryDNA, use microarray-based genotyping that reads the human genome at only ~ 500,000 positions. Nebula Explore is a shallow whole-genome sequencing at an average coverage of 0.4x per base that results in ~ 1.3 billion sequenced bases out of ~ 6.4 billion bases in the human genome. We launched Nebula Explore to create an affordable entry to personal whole genome sequencing. This generates billions of short sequencing reads (~ 150 letters) that are stored in text files in the FASTQ format. Next-generation sequencing (NGS) is a high-throughput DNA sequencing technology that enables the reading of billions of DNA molecules in parallel. Methods that determine the letter sequence of DNA molecules are called sequencing. The four letters of DNA are adenine (A), thymine (T), cytosine (C), and guanine (G). DNA is a chain-like molecule of variable length made of four building blocks, commonly called letters. DNA is a molecule that encodes the blueprint of every living organism.
0 kommentar(er)
